WGS of Bacteroides bacterium, strain NM69_E16B: murine gut isolate - SRA

SRX5766033: WGS of Bacteroides bacterium, strain NM69_E16B: murine gut isolate
1 ILLUMINA (Illumina NovaSeq 6000) run: 18.5M spots, 5.2G bases, 1.5Gb downloads

Design: Sequencing performed by Dr. Brian Yu at the Genomics Platform of the Chan Zuckerberg Biohub. Nextera XT kits were used to make the libraries with CZ Biohubs custom designed adapters - 12 bp index sequences on each side of the library and are dual unique so that each sequence only appears once. The libraries were made in 384 well plates in 4 uL of total volume. Genomic DNA was diluted to 0.18 ng/uL and 0.4 uL was added to the library prep reaction using Mosquito HTS from TTP. 0.5 uL of the library was pooled for sequencing. Sequencing was performed on a NovaSeq S4 flow cell with the run configuration 140 (12) (12) 140, so thats 2x140 bps with dual 12 bp index sequences.

Submitted by: University of Toronto

Study: Genome sequencing and assembly - library of cultured bacterial species from laboratory mouse gut microbiota from the University of Toronto

PRJNA474907 • SRP193474 • All experiments • All runs

show Abstracthide Abstract

Bank of bacterial mono-isolates from laboratory mouse gut microbiota

Sample:

SAMN10878311 • SRS4700349 • All experiments • All runs

Organism: Bacteroides muris (ex Afrizal et al. 2022)

Library:

Name: NM69_E16B

Instrument: Illumina NovaSeq 6000

Strategy: WGS